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Gus assay mature leaves

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A selectable marker is a gene introduced into a cell, especially a bacterium or to cells in culture, that confers a trait suitable for artificial selection. Transient expression profiles for several chimeric -glucuronidase (gus) gene constructs were determined in tissues (young leaves, mature leaves and roots) of creeping bentgrass (agrostis palustris, cv. The constructs analyzed consisted of the uida (gus) reporter gene driven by four different promoters (ubiquitin 3-potato, ubiquitin corn.). Results suggest that the ubiquitin rice promoter is most active in cells of turfgrass, regardless of the developmental stage or tissue-type. Of the four promoter used, except for ubiquitin 3-potato, reporter gene activity was dramatically higher in mature leaves compared to young leaves. Agroinfiltration assays showed gus transient expression in leaves of cassava varieties widely cultivated in the north coast and eastern savannah, mcol2215 (venezuelan) and cm6438-14 (vergara.). Histochemical localization of gus activity in transgenic plants was carried out essentially as described by jefferson et al. Plant material was incubated in a gus assay buffer 10 mm na 2 edtah 2 0, 0. In a fluorometric gus assay, the two promoters showed similar activities in stems, mature leaves and female flowers while the camv35s promoter was more effective than the jcuep promoter in other tissues, especially young leaves and inflorescences. In addition, the jcuep promoter retained its activity under stress conditions in low temperature. To high levels in late-developing embryos or fresh mature seeds as is typical of lea gene. That from mature leaves (figure 2), indicating that rice pcna mrna was specifically expressed in rapidly cell-dividing organs of rice plants. Fluorometrical gus assay gus assay with protein extracts of mature and immature leaves from transgenic plants was carried out using the fluorometric assay procedure described by jefferson et al. (d) histochemical gus assay of mature leaves from untransformed control plant and t1 plants transformed with the 35sgus (pcambia1201) construct. (eg) expression of the gfp reporter gene in three dpg seedling, seven dpg plantlets and 10 dpg leaf of t1 lines transformed with the 35sgfp (ph7m24gw35spgfp) construct.

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